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. 2007 Oct 19;8(11):1068–1073. doi: 10.1038/sj.embor.7401094

Figure 2.

Figure 2

The H19 imprinting control region produces sense and antisense transcripts. Reverse transcription reactions with RNA obtained from mouse embryonic day (E)15.5 fetal liver cells, using primers specific for the sense (A) or antisense (E) ICR transcripts. Complementary DNA synthesis reactions were carried out with or without reverse transcriptase (RT), and with or without strand-specific primers. PCR products correspond to amplicons 4 (H19 ICR) and 7 (H19 silencer) in Fig 1. Sequence traces of PCR products are shown below the gel pictures, with asterisks indicating SNPs. Detection of Igf2 in combination with ICR sense (B–D) and antisense (F–H) transcripts in mouse E14.5 fetal liver cells by double-labelled RNA-FISH. Green signals are ICR transcripts, red signals are Igf2 gene transcripts. DAPI staining is blue. Bars below the pictures show frequencies of the respective signals. DAPI, 4,6-diamidino-2-phenylindole; FISH, fluorescence in situ hybridization; ICR, imprinting control region; SNP, single-nucleotide polymorphism.