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. 2007 Oct 19;8(11):1068–1073. doi: 10.1038/sj.embor.7401094

Figure 3.

Figure 3

RNA interference-mediated downregulation of H19 imprinting control region non-coding RNA leads to loss of reporter gene silencing. (A) Quantification of reporter gene expression by photometric measurement of eye colour pigmentation in Drosophila lines carrying the transgene combinations indicated below the respective bars. (B) Shown here are the eyes of the Drosophila lines carrying the H19 ICR transgene (left panel), the H19 ICR transgene combined with the RNAi vector (middle panel), and the H19 ICR transgene combined with the RNAi vector and GAL4 (right panel). (C) Reverse transcription (RT) reaction with primers in the H19 ICR with (+RT, upper panel) or without (−RT, lower panel) reverse transcriptase. RNA samples are obtained from heads of the respective Drosophila lines shown in (B). (D) Reverse transcription reaction with primers against β-tubulin. RNA samples used for the reverse transcription reaction are the same as in (C). ICR, imprinting control region; OD, optical density; RNAi, RNA interference.