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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Jan;49(1):197–203. doi: 10.1167/iovs.07-1038

Figure 3.

Figure 3

Mucin O-glycans contribute to the prevention of epithelial cell surface adhesion under static conditions. Fluorescein-labeled HCLE cells and human fibroblasts in suspension showed reduced binding to HCLE cells grown under conditions that promote mucin O-glycan biosynthesis (Serum), compared with serum-free media (SFM) or to cells treated with the inhibitor benzyl-α-GalNAc (Serum+BG). In control experiments, fluorescein-labeled fibroblasts showed similar levels of adhesion to fibroblasts grown in the presence (FGM+BG) or absence (FGM) of benzyl-α-GalNAc, indicating that benzyl-α-GalNAc influences cell adhesion through the inhibition of O-glycosylation in mucin-expressing cells. Error bars, SEM.