Fig. 1.

gp70-pulsed DCs inhibit established CT26 lung metastases. gp70 or β-gal peptide was pulsed onto DCs pretreated with CD40L-3T3 cells or control 3T3 cells. DCs were then administered to mice bearing 3-day lung metastases. Twelve days later lungs were removed and metastases enumerated. Mice receiving gp70-pulsed DCs had significantly fewer lung metastases than mice receiving HBSS or β-gal-pulsed DCs (P2 < 0.01). An additional significant reduction in the number of lung metastases was seen after treatment of DCs with CD40L before pulsing with gp70. Data from two experiments are presented here with experiment 1 represented by □ and experiment 2 by ●.