Table 1. Immunization with vaccinia gp70 generates gp70 peptide-reactive splenocytes.
Splenocytes were harvested from mice 4 weeks after immunization with either vaccinia gp70 or vaccinia β-gal and restimulated in vitro with gp70 peptide. After 6 days splenocytes were assessed for IFN-γ secretion (pg/ml) in response to the targets listed. Splenocytes from mice immunized with vaccinia gp70 secreted high amounts of IFN-γ specifically in response to incubation with gp70 peptide pulsed splenocytes.
Targets |
|||||
---|---|---|---|---|---|
Immunogen | None | Fresh splenocytes | gp70-pulsed splenocytes | P1A-pulsed splenocytes | β-gal-pulsed splenocytes |
Vaccinia β-gal | 1343 | 2088 | 2708 | 1978 | 3555 |
Vaccinia gp70 | 1208 | 1536 | 20,543 | 1048 | 9526 |