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. 1998 Apr 14;95(8):4253–4257. doi: 10.1073/pnas.95.8.4253

Figure 1.

Figure 1

Effect of subtilisin on vinblastine-induced tubulin polymerization. Rat brain tubulin (2 mg/ml) was polymerized at 25°C, with 10% dimethyl sulfoxide and 50 μM vinblastine. Curves: 1, control, 1 mM GTP added at arrow; 2, with 0.5 mM GTP, subtilisin (1:100) added at thick arrow; 3, subtilisin added at beginning, left arrow indicates 1 mM GTP was added, and right arrow indicates 1 mM sodium triphosphate was added; 4, with 0.5 mM GTP and subtilisin added at beginning.