Figure 2.

Template properties of the degenerate bases. An oligonucleotide template containing unnatural bases, represented by X, and the two primers used to amplify, by PCR, the template are shown at the top; two columns of sequences showing only the regions between the two primers are shown below. The top row of each column of sequences shows the relevant portion of the oligonucleotide template (underlined sequence), on the left, are the sequences of 16 clones copied from a template containing six P residues, and on the right, are those copied from a template containing six K residues.