Figure 3.

eIF4G degradation is induced upon entry into the diauxic phase. (A) Strain CWO4 was allowed to grow to the diauxic phase (points 2 and 3), diluted into fresh medium, and allowed to resume exponential growth (points 4–6). Strain CBY1.1 (TIF4631 deletion) was allowed to grow to the diauxic phase (points c and d). Time points indicated (1–6) correspond to CWO4 cells harvested at a cell density (OD₆₀₀) of 1.302 (time point 1), 4.66 (time point 2), 6.6 (time point 3), 0.178 (time point 4), 0.445 (time point 5), and 1.10 (time point 6) and to CBY1.1 cells with an OD₆₀₀ of 1.81 (time point a), 3.29 (time point b), 6.19 (time point c), and 6.88 (time point d). (B) Flow cytometry analysis (graphs) of CWO4 cells harvested at time points 1–6 in A. (C) Western blots of extracts from CWO4 cells harvested at time points 1–6 (lanes 1–6) and CBY1.1 (lanes a–d) and decorated with antibodies against eIF4G, eIF4A, and eIF4E.