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. 1998 Apr 14;95(8):4282–4287. doi: 10.1073/pnas.95.8.4282

Figure 4.

Figure 4

(A) Excitation spectra of AEDANS-Cys-509/Trp-424 single Trp mutant of P178. Spectra: a, P178s; b, P178m. Both were in 20 mM dimethylglutaric acid, pH 4.0/0.1 M NaCl at 2°C. Emission was measured at 490 nm. Liposomes contained a DOPG/DOPC molar ratio of 2:3. P178 and lipid concentrations were 0.2 μM and 0.1 mM, respectively. (B) Temperature (T) dependence of FRET efficiency for P178s (trace a) and P178m (trace b). Two measurements with increasing T are shown: P178s, (▵) T increased to 50°C, decreased to 15°C, followed by a second scan (○); P178m (×), 10°C → 50°C, followed by a second scan (□). Heating rate, 1.5–2.0°C/min.