Table 1.
Postnatal retinal cell genesis as quantified by thymidine birthdating and either immunofluorescent or histological identification of cell fate
| P0 | P2 | P4 | P6 | |||||
| Cell type | IF | Hist* | IF | Hist* | IF | Hist* | IF | Hist* |
| Bipolar | 2.19 ± 1.45 | 6.51 | 3.98 ± 2.66 | 17.04 | 12.81 ± 6.16 | 31.81 | 16.01 ± 6.31 | 25.74 |
| Cone type | 1.67 ± 1.15 | NA | 2.73 ± 1.90 | NA | 7.96 ± 3.23 | NA | 8.20 ± 2.97 | NA |
| Rod type | 0.52 ± 0.32 | NA | 1.21 ± 1.01 | NA | 4.85 ± 3.35 | NA | 7.82 ± 3.41 | NA |
| Amacrine | NA | 9.16 | NA | 0.05 | NA | 0.00 | NA | 0.00 |
| Rod | 69.69 ± 12.65 | 81.67 | 85.73 ± 4.15 | 79.51 | 71.22 ± 5.88 | 59.54 | 62.74 ± 8.63 | 60.59 |
| Muller | 0.68 ± 0.26 | 2.66 | 0.58 ± 0.43 | 3.40 | 2.73 ± 1.08 | 8.65 | 4.04 ± 1.97 | 13.67 |
Postnatal mice were injected with tritiated thymidine at the developmental time indicated. For the immunofluorescent (IF) methodology, retinae were harvested at P16, dissociated and stained using established cell-type specific markers, namely anti-Chx10 (bipolar cells), Rho4D2 (rod photoreceptors), and anti-glutamine synthetase (Muller cells). *For the histochemical (Hist) estimates all data are based on [19]. Cells born from the central and peripheral sections were summed to thereafter derive a percentage of each cell type