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. 2007 Nov 13;8:29. doi: 10.1186/1471-2172-8-29

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Copyright © 2007 Elshal et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mononuclear cells from the peripheral blood of a normal volunteer were incubated with carboxyfluorescein diacetatesuccinimidylester (CFSE) and PHA, as described in the text. CFSE fluorescence reduced with each cellular division. The cells were cultured a total of five days. The upper left panel displays CFSE staining versus a PE isotype control of CD3+ cells. The upper right panel shows staining of PECD146 versus CFSE of CD3+ T cells. Lower panels display CFSE vs CD4 and CD8. CFSE staining decreased with each successive division of the T cells, and CD146 expression increased.