Figure 6.

Effect of PAO on uptake of LDL by PAEC grown under static conditions. The glycocalyx layer is visualized under the confocal microscope through staining of heparan sulfate with FITC-conjugated lectin (green) and LDL added as DiI complex (red) after different times in culture. (a) Cells grown under static conditions for 24 hours and incubated with PAO together with FITC-conjugated lectins and (b) cells grown under static conditions for 24 hours. Internalized LDL appear yellow when colocalized with heparan sulfate stain. (c) Cross-sections of image stacks created with confocal microscopy of (a) and (d) cross-sections from the side of the stack of images of (b).