FIGURE 4.

The translational repressor domain of dUNR is embedded within its amino-terminal 397 amino acids. The translation inhibition of BmLMS2 mRNA by increasing amounts of tethered MS2-UNR derivatives was measured. The result obtained at a 30-fold molar excess of protein to RNA is shown. The confidence between critical values are depicted (lanes 3,4, P = 0.027; lanes 3,6, P = 0.001; lanes 8,10, P = 0.035). Similar results were obtained when BLMS2 mRNA was used (data not shown). A schematic representation of the different constructs is shown at the left. Proteins were expressed as His-tagged MS2 fusions.