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. 2008 Mar;14(3):491–502. doi: 10.1261/rna.665008

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FIGURE 6. — Myo2p associates with P-body components. (A) Differential sedimentation of lysates from cells expressing TAP-tagged P-body subunits Dcp1p and Lsm1p. (B) Optiprep density gradient fractionation of P3 isolated from cells expressing TAP-tagged Dcp1p or Lsm1p. (C) Coprecipitation of Myo2p with TAP-tagged Lsm1p. P3 from cells expressing TAP-tagged Lsm1p was mixed with either BSA- or mouse IgG-coated magnetic beads. The pellet and supernatant fractions were analyzed by immunoblotting. (D) Dhh1-GFP coprecipitates with Myo2p pulled down by anti-myo2-head but not anti-myo2-tail antibody. Gradient and IP fractions were immunoblotted with anti-Myo2p or anti-GFP antibodies or rabbit IgG to visualize the TAP tag. The pellet fractions were loaded at twofold (2×) or threefold (3×) concentrations of the supernatant fractions.