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. 2008 Feb 21;118(3):868–878. doi: 10.1172/JCI33160

Figure 2. Curcumin suppresses p300-induced hypertrophic responses in cardiomyocytes.

Figure 2

(A and B) Cardiomyocytes were transfected with 0.7 μg of pCMVwtp300 (lanes 2 and 3) or pCMVβ-gal (lane 1) and incubated with curcumin (10 μM, lane 3) or a corresponding amount of its vehicle (lanes 1 and 2) for 48 hours. (A) Protein extracts from these cells were subjected to Western blotting using the anti-p300, anti-GATA4, and anti–β-actin antibodies. (B) Cell-surface area was measured as described in Methods. Values in each group are mean ± SEM (μm) from 50 cells. Cardiomyocytes were cotransfected with 0.5 μg of pANF-luc (C) or pβ-MHCluc (D), 0.0025 μg of pRL-SV40, and 0.25 μg of pCMVwtp300 (lanes 2 and 3) or pCMVβ-gal (lane 1). Then, these cells were incubated with curcumin (10 μM, lane 3) or its vehicle (DMSO, lanes 1 and 2) for 48 hours. The relative promoter activities were calculated from the ratio of firefly Luc to sea pansy Luc and expressed as the mean ± SEM from 3 independent experiments, each carried out in duplicate. (E) Cardiomyocytes were transfected with 0.7 μg of pCMVwtp300 (bars 2 and 3), pCMVHATmutp300 (bars 4 and 5), or pCMVβ-gal (bars 1, 6, and 7) and incubated with TSA (bars 6 and 7), curcumin (10 μM, bars 3, 5, and 7), or a corresponding amount of their vehicles for 48 hours. Cell-surface area was measured as described in Methods. Values in each group are mean ± SEM (μm) from 50 cells.