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. 2008 Feb 21;118(3):956–964. doi: 10.1172/JCI34314

Figure 4. ChREBP, but not LXR, is required for glucose-regulated gene expression.

Figure 4

(A) ChREBP protein in nuclear extracts from wild-type and LXRα/β knockout hepatocytes. A representative Western blot is shown (n = 4 per group). (B) qRT-PCR analysis of L-PK, ACC, LXRα, and ABCA1 in isolated hepatocytes from wild-type and LXRα/β knockout mice. Hepatocytes from both genotypes were incubated under low glucose (5 mM) plus 100 nM insulin in the presence of a scramble siRNA or under high glucose concentrations (25 mM) plus 100 nM insulin in the presence of either scramble or ChREBP siRNA (8) for 24 h. Error bars represent SD (n = 4 independent cultures). *P < 0.005 vs. 5 mM glucose plus scramble siRNA and 25 mM glucose plus ChREBP siRNA groups. n.d., not detectable. (C) Total ChREBP protein in lysates from wild-type and LXRα/β knockout murine hepatocytes transfected with either scramble or ChREBP siRNA. A representative Western blot is shown. n = 4 independent cultures.