TABLE 4.
Mutation rates in strains with different chromosomal dpb2 alleles and in dpb2 msh6 double mutants
| Mutation ratesb,c
|
||||
|---|---|---|---|---|
| DPB2 allelesa | CanR/107 | His+/108 | Trp+/108 | Lys+/108 |
| DPB2 | 5 (±1) [1] | 5 (± 5) [1] | 5 (±5) [1] | 8 (±6) [1] |
| dpb2-100 | 36 (±12) [7] | 97 (±38) [19] | 18 (±12) [4] | 44 (±3) [6] |
| dpb2-101 | 18 (±4) [4] | 30 (±7) [6] | 13 (±8) [3] | 23 (±9) [3] |
| dpb2-103 | 25 (±6) [5] | 53 (±21) [11] | 24 (±13) [5] | 55 (±22) [7] |
| msh6 DPB2 | 47 (±12) [9] | 13 (±6) [3] | 18 (±4) [4] | 12 (±7) [1.5] |
| msh6 dpb2-100 | 222 (±53) [44] | 175 (±74) [35] | 150 (±60) [30] | 254 (±44) [32] |
| msh6 dpb2-101 | 191 (±22) [38] | 78 (±9) [16] | 145 (±52) [29] | 213 (±41) [27] |
| msh6 dpb2-103 | 235 (±74) [47] | 125 (±6) [25] | 139 (±69) [28] | 237 (±12) [30] |
a
dpb2 alleles integrated into the DPB2 chromosomal locus of ΔI(-2)I-7B-YUNI300 and ΔI(-2)I-7B-YUNI300 msh6∷hisG, respectively.
b
“±” indicates standard deviations from three experiments.
c
Within brackets are folds presenting the increase of mutability (the rate of mutagenesis in the respective dpb2 mutant is divided by the rate of mutagenesis in the strain carrying the wild-type DPB2 gene in the MSH6 background).