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Effects of two daf-2 mutations on DAF-16 localization. (A) daf-2(+) at 25°. (B) daf-2(e1370) at 25°. (C) daf-2(m577) at 25°. (D) daf-2(e1370) at 15°. Anterior of animal to the right. Arrows indicate nuclear localization of DAF-16∷GFP. Arrowheads indicate position of the grinder of the pharynx. Bar, 50 μm.
