Figure 1.—

Phenotypic analysis of the cell wall stress reporter strain. Strain RD6.47, containing both the nuclear targeted GFP (H2B-GFP) and acetamidase (amdS) reporters under control of the agsA promoter, was grown for 3 days at 30° on plates containing acetamide as the sole nitrogen source under normal growth conditions (A) and with the addition of 0.5 mg ml⁻¹ CFW giving an induction of the reporter (B). The reporter strain was also allowed to germinate on submerged cover slides. After 5 hr of growth at 37°, germlings were treated with 200 μg ml⁻¹ CFW (D and F) or an equal amount on MilliQ water (C and E) and grown for another hour before microscopic observation. C and D show bright field; E and F, fluorescence microscopy.