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DAF-16∷GFP is localized to the nucleus in animals treated with RNAi clones that cause sterility. Shown are representative images of DAF-16∷GFP protein in intestinal cells of TJ356 animals fed with various RNAi clones including L4440 vector control (A and B), glp-1 (C and D), inx-14 (E and F), or fog-2 (G and H). Differential interference contrast images are in A, C, E, and G and GFP images are in B, D, F, and H. Only fully sterile animals were analyzed for DAF-16∷GFP.
