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. 2008 Feb;178(2):903–918. doi: 10.1534/genetics.107.083923

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Copyright © 2008 by the Genetics Society of America

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Figure 8.— — DAF-16-dependent genes are upregulated in adult sterile mutants. The expression of four DAF-16-dependent genes, mtl-1, sod-3, lys-7, and dod-6, in gravid adult animals (A) or young adult animals without embryos (B) was determined by qRT–PCR and compared to that of wild-type N2. Mutants analyzed were daf-2(e1368), daf-16(mgDf47), glp-1(e2141), fer-15;fem-1, fem-3(q20), and inx-14(ag17). DAF-16-dependent gene expression in 4-day-old fer-15;fem-1 adults was compared to 4-day-old wild-type N2 (C). Fold changes represent the average of at least three different biological samples. Error bars represent standard error of the mean.