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. Author manuscript; available in PMC: 2009 Feb 11.
Published in final edited form as: Behav Brain Res. 2007 Sep 2;187(1):48–55. doi: 10.1016/j.bbr.2007.08.036

Figure 1.

Figure 1

Generation of Nac1 mutant mice. A. The targeting vector of Nac1 a 5kb lacZ-PGK-neo cassette was inserted into exon 2; Enzyme restriction sites are designated as X;Xba and E; EagI, the positions of primersets used to detect wild-type and mutant alleles are indicated by black arrows, geneotypes of mice were identified using PCR primers corresponding to 5' and 3' in wild type allele, where the 3' primer is located just downstream of the EagI site where the targeting vector was inserted. B. DNA analysis in wild type and mutant mice. Wild-type band is 293bp, mutant band 340bp that is amplified using the same 5'primer and a primer located within the lacZ-PGK-neo cassette. C. The absence of Nac1 mRNA was confirmed by RT-PCR analysis using primer sets corresponding to either the POZ/BTB domain or a 5' region of Exon 1. HPRT is used as an internal control. D. The absence of Nac1 protein was confirmed in mutant mice by Western analysis in both nucleus accumbens (NAC-/-) and cortex (CTX-/-).