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. Author manuscript; available in PMC: 2008 Feb 20.
Published in final edited form as: Cancer Res. 1995 Jul 15;55(14):3149–3157.

Fig. 1.

Fig. 1

Flow cytometric analysis of allele-specific surface expression of HLA antigens in representative clones of 624-MEL. Data are presented as MCN of fluorescence. The following mAbs were used: MA2.1, anti-HLA-A2 and B17 (37); MB40.2, anti-HLA-B7, B40; 277-HA1, anti-HLA-B14; GAP A-3. anti-HLA-A3 (38). The original bulk culture (624-MEL) was HLA-A2, A3, -B7, B14 positive (first row) expressing the A loci in standard culture conditions (first two columns) and the B alleles only under stimulation with 500 units/ml of rIFN-γ for 48 h (last two columns). Clone 626.28 totally lost expression of HLA-A2 (second row), clone 624.23 has intermediate expression of HLA-A2 (third row), and clone 624.38 expresses the highest amount of HLA-A2 molecules on its cell surface (fourth row).