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. 2008 Feb 4;9:81. doi: 10.1186/1471-2105-9-81

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Copyright © 2008 Hamilton and Teasdale; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Visualizing the image set in 3 dimensions. (A) The 502 endogenous images visualized in 3 dimensions with coordinates determined from Sammon mapping Haralick and TAS measures. (B) 1407 cell images automatically cropped from the 502 images and visualized in 3 dimensions with coordinates determined from Sammon mapping Haralick and TAS measures. In both (A) and (B) images of each sub-cellular localization are strongly regionalized. In (A) note the clearly nuclear image center right with a lysosome (red border) classification. Possibly the DAPI nuclear image was incorrectly named when the image set was collected. Within the visualizer the user may zoom in/out, rotate, translate, click to select image classes to show/hide, show image names and visualize nearest neighbors (Figure 3). See also Additional file 2 and a simplified java script version available at [17].