Figure 1.

Rabbit aortic calcification occurred in the lower zone of thickened plaques adjacent to the media. The normal control contained neither thickened intima nor calcification in the media (not shown) [see Refs 7, 19]. Rabbits were fed an atherogenic diet containing 0.5% cholesterol and 2% peanut oil for 4 months. Segments of thoracic aortas were fixed with formalin, paraffin blocked, thin sectioned. A) H & E staining was used to show intimal thickening. B) Mineral deposits were visualized as alizarin red stains (AR).