Table 1.

List of identified proteins related to calcification in calcifying vesicles from tadem spectrometry. Data were analyzed using a Biowors 3.2 (ThermoFinnigan) program.

Proteins	Peptide Sequence
Calpactin I Heavy chain	SNAQRQDIAFAYQR (2, 4.50, 0.32) LSLEGDHSTPPSAYGSVK (2, 4.00, 0.40) RAEDGSVIDYELIDQDAR (2, 3.69, 0.29) GVDEVTIVNILTNR (1, 2.83, 0.29)
Calreticulin or CRP55	FYALSAR (1, 2.00, 0.44) FKPFSNKGQPLVVQFTVK (2, 3.75, 0.64) EPVVYFKEQFLDGDGWTER.G (2, 10.2, 0.54)
Integrin beta 1	SKGTAEKLQPEDITQIQPQQLVLQLR (1, 2.02, 0.36) GTAEKLQPEDITQIQPQQLVLQLR (2, 5.42, 0.54) LQPEDITQIQPQQLVLQLR (2, 5.42, 0.54) SGEPQTFTLK (2, 2.02, 0.36)
Fibrillin	IKGTQC#EDINEC#EVFPGVC#K (2, 3.00, 0.36) IKGTQC#EDIDEC#EVFPGVCKNGLCVNTR (3, 4.58, 0.35)
ATPase β subunit	VALTGLTVAEYFRDEEGQDVLLFIDNIFR (3, 2.70, 0.27) AIAELGIYPAVDPLDSTSR (2, 4.76, 0.12)
ATP synthase	ITSTKEGSITSIQAIYVPADDLTDPAPATTFAHLDATTVLSR (3, 5.69, 0.17) VLDSGAPIKIPVGPETLGR (2, 3.81, 0.65) ILQDYKSLQDIIAILGMDELSEEDKLTVSR (3, 3.78, 0.55) ILGADTSVDLEETGRVLSIGDGIAR (2, 3.26, 0.19) TGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSK (2, 5.13, 0.47) AIAELGIYPAVDPLDSTSR (1, 3.0, 0.46)

The following set of criteria for high confidence were used: minimum cross-correlation score of (Xcorr) of 1.9, 2.5 and 3.5 for 1, 2 and 3 charges ions respectively, a delta correlation score (Δcorr) greater than 0.1, and a minimum of 2. These parameters are presented in the parentheses in sequence of charges, Xcorr, and Δcorr. Database used was obtained from the NCBI repository. C# indicates a carboxymethylcisteine.