Figure 5.

Dose-dependent changes in activity of the cellular retinol binding protein II (CRBPII) promoter in human kidney 293T cells induced by the CLOCK/BMAL1 gene (0–140 ng) plus the PPAR$\alpha$/RXR$\alpha$ gene (50 ng). The del-0-CRBPII-Luc plasmid contained no repeat sequences ($5^{\prime }$-AG(G/T)TCA-$3^{\prime }$); the del-1-CRBPII-Luc plasmid contained one repeat sequence ($5^{\prime }$-AG(G/T)TCA-$3^{\prime }$); the del-2-CRBPII-Luc plasmid contained two repeat sequences ($5^{\prime }$-AG(G/T)TCA-$3^{\prime }$); the del-3-CRBPII-Luc plasmid contained three repeat sequences ($5^{\prime }$-AG(G/T)TCA-$3^{\prime }$), and the del-4-CRBPII-Luc plasmid contained four repeat sequences ($5^{\prime }$-AG(G/T)TCA-$3^{\prime }$). The luciferase activity of all pCRBPII-Luc plasmids was normalized to the luciferase activity of pRL-TK. Experiments were performed in triplicate, and four independent experiments were performed. All data are means ± SD.