Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Mar 5;3(3):e1719. doi: 10.1371/journal.pone.0001719

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Ye et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

The insert fragments of the VEGF 3′-UTR in pRL-VEGF-Con1 and/or pRL-VEGF-Con2 were changed to fragments of the c-Met 3′-UTR or COX-2 3′-UTR to create pRL-CMET-Con3 and pRL-COX2-Con4. These changes allowed us to compare miRNA:MRE duplexes bearing standard central loops (A and D) in miRNA:MRE duplexes with the ones bearing Type I (B) and Type II (E) decentered loops. Luciferase activity assays indicated that changes to Type I and Type II decentered loops decreased the repressive effect of miRNAs significantly (C and F).