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. Author manuscript; available in PMC: 2008 Feb 21.
Published in final edited form as: J Biol Chem. 2005 Nov 11;281(2):1128–1136. doi: 10.1074/jbc.M510975200

FIGURE 3. Western blot analysis of P. aeruginosa 1244 mutant pilins.

FIGURE 3

Western blot of 1244 pilin produced by P. aeruginosa 1244N3/p1244 S148T and 1244N3/p1244 A149 of cell extracts using anti-1244 pilin monoclonal antibody 5.44 (A) or anti-1244 pilin glycan monoclonal antibody 11.14 (B) as a probe. G is glycosylated 1244 pilin, and NG is nonglycosylated 1244 pilin. Pilin terminating in Thr rather than Ser was glycosylated (1244N3/p1244 S148T), whereas pilin terminating in Ala, where Ser148 was the penultimate residue, was nonglycosylated.