Skip to main content
. Author manuscript; available in PMC: 2008 Feb 21.
Published in final edited form as: J Biol Chem. 2005 Nov 11;281(2):1128–1136. doi: 10.1074/jbc.M510975200

FIGURE 5. Western blot analysis of P. aeruginosa hybrid pilin.

FIGURE 5

Western blot of pilin produced by P. aeruginosa 1244.47/pHYBRID and 1244.47/pHYB-O using anti-pilin polyclonal serum as a probe (A) or anti-1244 pilin glycan monoclonal antibody 11.14 (B) as a probe. G is glycosylated 1244 pilin, and NG is nonglycosylated 1244 pilin. Hybrid pilin was glycosylated in the presence of PilO (pHYBRID), whereas removal of PilO abrogated glycosylation (pHYB-O). The presence of a pilin band was found only in the presence of IPTG (A), and empty vector controls did not produce a band (pMMB66EH).