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. Author manuscript; available in PMC: 2008 Feb 21.
Published in final edited form as: J Biol Chem. 2005 Nov 11;281(2):1128–1136. doi: 10.1074/jbc.M510975200

FIGURE 6. Western blot analysis of P. aeruginosa 1244 mutant pilins with Cys mutation or DSL deletion.

FIGURE 6

Western blot of pilin produced by P. aeruginosa 1244N3/p1244 C127A and 1244N3/p1244 C145A using anti-1244 pilin monoclonal antibody 5.44 as a probe (A). Western blot analysis of pilin produced by P. aeruginosa 1244N3/p1244Δ135–139, 1244N3/p1244Δ135–144, and 1244/p1244Δ128–144 using anti-1244 pilin monoclonal antibody 5.44 as a probe (B) or anti-1244 pilin glycan monoclonal antibody 11.14 (C) as a probe. G is glycosylated 1244 pilin, and NG is nonglycosylated 1244 pilin. All of the mutants produced some glycosylated pilin; however, p1244 C127A (A), p1244Δ135–144, and p1244Δ128–144 (B) produced some nonglycosylated pilin.