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. Author manuscript; available in PMC: 2008 Feb 21.
Published in final edited form as: J Biol Chem. 2005 Nov 11;281(2):1128–1136. doi: 10.1074/jbc.M510975200

FIGURE 8. Western blot analysis of PA103 mutant pilin with insertion.

FIGURE 8

Western blot of pilin produced by P. aeruginosa 1244.47/p103 INS136 in the presence of PilO (pUCP26pilO) or without PilO (pUCP26) using anti-pilin polyclonal serum as a probe (A) or anti-1244 pilin glycan polyclonal serum (B) as a probe. G is glycosylated 1244 pilin, and NG is nonglycosylated 1244 pilin. Pilin encoded by p103 INS136 was glycosylated in the presence of PilO, whereas removal of PilO abolished glycosylation. The presence of a pilin band was consistent with the presence of IPTG (A), and empty vector controls did not produce a band (pMMB66EH).