Table 2.
Preliminary characterization of rrl mutants
| MIC (ng/ml) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Strain | rrl Genotype | tgen (min) | Amp | Nov | Rif | Cam | Ery | Kan | Tet |
| RS526 | WT | 39 | 2 | 0.5 | 4 | 1 | 2 | 4 | 0.5 |
| RS537 | Δ1-rrIG | 48 | 2 | 0.5 | 4 | 1 | 2 | 4 | 0.5 |
| RS544 | Δ2-rrlGH | 58 | 2 | 0.5 | 4 | 1 | 2 | 8 | 0.5 |
| RS547 | Δ3-rrlDGH | 59 | 2 | 0.5 | 4 | 1 | 2 | 8 | 1 |
| RS550 | Δ4-MDEGH | 57 | 2 | 0.5 | 4 | 1 | 4 | 4 | 1 |
| RS554 | Δ5-MBDEGH | 60 | 1 | 0.5 | 4 | 1 | 4 | 4 | 1 |
| RS676 | Δ6-rrlABEDGH | 87 | 1 | 0.5 | 4 | 0.5 | 4 | 4 | 0.5 |
Bacterial strain numbers are listed in column one and their genotypes with respect to 23S rRNA genes are shown in column two (please refer to Table S1 for complete genotype). Column three shows doubling time in LB media at 37°C (tgen in minutes). Minimum inhibitory concentration (µg/ml) of known antibacterial agents are shown for each strain tested in microtiter plates in LB broth at 35°C. Amp: ampicillin; Nov: novobiocin; Rif: rifampicin; Cam: chloramphenicol; Ery: erythromycin; Kan: kanamycin; Tet: tetracycline. Other agents tested but not shown: Linezolid, Puromycin, Azithromycin, Kasugamycin, Spectinomycin, Fusidic Acid, and Kirromycin.