Figure 4.
The observation of the protein-ssDNA complexes by electron microscopy. (A, G) The images of SSB in the presence of M13 ssDNA. (B, C) The images of RecO in the presence of M13 ssDNA. (D) The image of SSB-RecO in the presence of M13 ssDNA. M13 ssDNA was incubated with SSB for 10 min, and then RecO was added to the solution. (E) The image of RecO-SSB in the presence of M13 ssDNA. M13 ssDNA was incubated with RecO for 10 min, and then SSB was added to the solution. (F) The wide-ranging image of SSB-RecO in the presence of M13 ssDNA. The arrowheads indicate the protein-ssDNA complexes. Scales of each image are indicated by the white bars. Parentheses indicate the classified shapes in Table 1 (a, b and d).