Figure 8.

The effect of RecR on the ssDNA-binding activity of RecO. (A) The indicated concentrations of RecO were incubated with 10 μM εDNA at 25°C for 5 min in the absence (circle) or presence (triange) of 10 μM RecR. The effect of RecR alone is represented by a cross. The measurement conditions were the same as described for Figure 4. (B) The indicated concentrations of RecO were incubated with 10 μM εDNA at 25°C for 5 min in the presence of 10 μM RecR_E144A (square). The effect of RecR_E144A alone (0–5 µM) is represented by a plus, and the effect of 10 μM RecR_E144A is shown by a bracketed plus. The reverse triangle represents the binding curve of RecO to ssDNA with the ΔF of the 10 μM RecR_E144A alone (∼35) subtracted. (C) An agarose gel retardation assay of the RecO-RecR complex. A 5′-³³P labeled 66-mer ssDNA was incubated with the indicated concentrations of RecO and RecR for 5 min at 50°C, and then the samples were analyzed by electrophoresis on a 2.5% agarose gel.