Figure 1.
(A) Summary of the sequence of events performed by ExEnSo. (B) Sequence alignment of the mutable region (−17/+9) of the 10 sequences optimized by ExEnSo. S, sequence of the standard forward primer. 1 to 10, coding sequences used in this study (for details, see Supplementary Figure 1). On top of the alignment are indicated the mutable bases (N1 to Y8) and the translation initiation codon (ATG). Grey boxes contain the mutated bases.