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. 2007 Nov 5;36(1):67–75. doi: 10.1093/nar/gkm943

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — p21 inhibits recruitment of MMR proteins and PCNA to replicating DNA. In panel (A) p21C (34 µg/ml) was added to the SV40 DNA replication reaction. In panel (B) p21C (34 µg/ml) and exogenous PCNA (30 µg/ml) were added to the SV40 DNA replication reaction. After gel filtration, precipitated protein fractions were analyzed by immunoblotting for MSH2, RPA70 and PCNA as above. As a control for antibody recognition, lanes marked CE contained 5 µl of cell extract representing 7% of the proteins in the entire reaction.