Table 2.
Transcriptional targets of p110 CUX1 that play a role in cell cycle
| Function | Gene symbol | Gene description | ChIP/qPCR: Fold/total | |
|---|---|---|---|---|
| Unsync. | Thymidine | |||
| Cell cycle, S phase | CCNA2 | Cyclin A2 | 1.6 | 5.1 |
| CDC25A | Cell division cycle 25A | 2.5 | 7.5 | |
| Cell cycle, G2/M | CDC25B | Cell division cycle 25B | 2.1 | 5.6 |
| CDC25C | Cell division cycle 25C | 0.7 | 3.2 | |
| MAD2L1 | MAD2 mitotic arrest deficient-like 1 (yeast) | 1.2 | 3.7 | |
| Cell cycle, M Phase | ANAPC4 | Anaphase-promoting complex subunit 4 | 2.9 | 4.8 |
| APC10 | Anaphase-promoting complex subunit 10 | 1.8 | 2.6 | |
| EML4 | Echinoderm microtubule-associated protein-like 4 | 2.6 | 4.0 | |
| FNBP4 | Formin-binding protein 4 | 1.7 | 14.1 | |
| FZR1 | Fzr1 protein | 3.7 | 24.6 | |
| KIF11 | Kinesin family member 11 | 2.0 | 8.0 | |
| KNSL7 | Kinesin-like 7 | 1.8 | 4.7 | |
| KNTC1 | Kinetochore-associated 1 | 0.7 | 7.9 | |
| LATS1 | Large tumor suppressor 1 | 5.0 | 11.8 | |
| NUMA1 | Nuclear mitotic apparatus protein 1 | 1.9 | 2.2 | |
| PRC1 | Protein regulator of cytokinesis 1 | 3.6 | 5.5 | |
| SKB1 | SKB1 homolog (S. pombe) | 1.8 | 4.7 | |
| SMC4L1 | SMC4 structural maintenance of chromosomes 4-like 1 | 3.5 | 8.0 | |
| TOPK | T-LAK cell-originated protein kinase | 3.5 | 3.4 | |
| Cell cycle, meiosis | STAG2 | Stromal antigen 2 | 9.2 | 2.5 |
| DNA replication | CDC45L | CDC45 cell division cycle 45-like (S. cerevisiae) | 0.8 | 9.0 |
| CDC7 | CDC7 cell division cycle 7 (S. cerevisiae) | 2.1 | 5.4 | |
| CHAF1A | Chromatin ssembly factor 1, subunit A (p150) | 1.4 | 5.9 | |
| DNTT | Deoxynucleotidyltransferase, terminal | 1.2 | 4.0 | |
| DTYMK | Deoxythymidylate kinase (thymidylate kinase) | 2.1 | 10.3 | |
| MCM3 | MCM3 minichromosome maintenance deficient 3 | 2.8 | 13.0 | |
| MCM7 | MCM7 minichromosome maintenance deficient 7 | 1.9 | 5.3 | |
| MYST2 | MYST histone acetyltransferase 2 | 2.9 | 14.6 | |
| ORC1L | Origin recognition complex, subunit 1-like (yeast) | 2.0 | 7.2 | |
| ORC3L | Origin recognition complex, subunit 3-like (yeast) | 1.6 | 2.1 | |
| POLA | Polymerase (DNA-directed), alpha | 3.0 | 13.2 | |
| POLA2 | Polymerase (DNA-directed), alpha (70 kDa) | 7.2 | 21.3 | |
| POLD2 | Polymerase (DNA-directed), delta 2, | 0.6 | 6.3 | |
| POLD3 | Polymerase (DNA-directed), delta 3, accessory subunit | 2.9 | 17.0 | |
| RPA3 | Replication protein A3, 14 kDa | 1.9 | 7.9 | |
| Proliferation | CCNH | Cyclin H | 2.4 | 8.1 |
| EGF | Epidermal growth factor | 2.0 | 4.5 | |
| MVP | Major vault protein | 3.5 | 13.6 | |
| PURA | Purine-rich element-binding protein A | 0.9 | 5.1 | |
| Repair, checkpoint | ATR | Ataxia telangiectasia-related | 1.9 | 3.9 |
| CCNG2 | Cyclin G2 | 2.9 | 13.1 | |
| CHES1 | Checkpoint suppressor 1 | 2.2 | 22.4 | |
| MTBP | Mdm2 binding protein | 2.6 | 3.2 | |
| TP53 | Tumor protein p53 (Li-Fraumeni syndrome) | 1.9 | 6.0 | |
| Repair, homologous | RAD51 | RAD51 homolog (RecA homolog, E. coli) | 2.9 | 17.0 |
| Repair, mismatch | MLH1 | MutL homolog 1, colon cancer, nonpolyposis type 2 | 1.1 | 5.2 |
| MSH6 | MutS homolog 6 (E. coli) | 3.5 | 13.6 | |
| PMS1 | PMS1 postmeiotic segregation increased 1 | 2.9 | 6.3 | |
| PMS2L5 | Postmeiotic segregation increased 2-like 5 | 1.2 | 3.7 | |
The targets are organized in functional classes (column 1). Indicated are the gene symbols (column 2), the enrichment fold in conventional ChIP assays performed with HeLa cells, either unsynchronized (column 4) or submitted to a thymidine block (column 5). Enrichment of target promoters was calculated using the G6PDH locus as a reference and is shown relative to the chipped DNA obtained by immunoprecipitation with no antibody.