Table 3.

‘ATCRAT’ motif is over-represented in CUX1 cell cycle target genes

Motifs		ATCRAT	CCAAT	CRAT/CRAT	CRAT/GYTA
Percentage of promoters with motif(s)	Targets	52%**	90%	17%	15%
	Non-targets	13%	87%	15%	11%
Number of motifs per promoter	Targets	0.71**	2.40	0.21	0.15
	Non-targets	0.15	1.91	0.16	0.11

The promoter regions of target (Table 2) and 50 non-target genes were analyzed for the presence of putative CUX1-binding sites using the MacVector software. Promoter regions were defined as the sequences that were spotted on the microarray plus 250 bp on each side. R = A or G; Y = T or C. The significance of the difference between Targets and Non-Targets for each motif was determined by Student's t-test. **P=0.01.