Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Nov 22;36(1):330–341. doi: 10.1093/nar/gkm1028

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Induction of the CIDE-A gene expression by 5-aza-dC. (A) HEK-293T, Hela and BEL-7404 cells were treated with 1 or 10 μM 5-aza-dC. Seventy-two hours after 5-aza-dC treatment, total RNA was isolated from treated or untreated cells and subjected to RT-PCR to amplify CIDE-A. The GAPDH gene was used as a positive control for RNA integrity. (B) Bisulfite sequencing to determine the methylation status of the critical CIDE-A promoter region after 5-aza-dC treatment. Circles enclosed by boxes represent the CpGs in the Sp1/Sp3-binding sites.