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. 2007 Nov 22;36(1):330–341. doi: 10.1093/nar/gkm1028

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Knockdown of Sp1 and Sp3 expression by siRNA reduces CIDE-A promoter activity. (A) Immunoblotting analysis of Sp1 and Sp3 protein levels in HEK-293T cells transfected with Sp1 and Sp3 siRNA. Control siRNA was used as negative control and Tublin as a loading control. (B) The effect of Sp1 or Sp3 siRNA on CIDE-A promoter activity detected by luciferase reporter assay. HEK-293T cells were transfected with Sp1 and Sp3 siRNAs individually or simultaneously and then transfected again with the luciferase reporter plasmid p(−137/−13) and p(−1556/−137). The activity of each promoter construct is shown as the luciferase activity relative to that of the pGL3-Basic vector. Values were normalized for transfection efficiency by cotransfection with the Renilla expression plasmid and are shown as means ± SD for three independent experiments.