Figure 3.

Physical interactions between SMAD3 and functional domains of NKX2.1. GAL4 or VP16 fused NKX2.1 and its various functional domains were used in mammalian two-hybrid assays to determine the site of interactions with SMAD3. Vp16 or Gal4 plasmids were used either alone or in combination with other expression vectors as control. Luciferase reading from combination of empty Vp16 and Gal4 plasmids were adjusted to unity and used for normalization of all experimental values. Representative results from three independent experiments are shown. Asterisks denote P < 0.05.