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. 2007 Nov 14;36(1):179–188. doi: 10.1093/nar/gkm871

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Localization of SMAD3 functional domains, which interact with NKX2.1. Panel A, mammalian two-hybrid assays were used to test the ability of GAL4-fused MH1, GAL4-fused MH2 or GAL4-fused LNKR domains of SMAD3 to interact with a full-length VP16-fused NKX2.1. Empty Gal4 plasmid was used as control and the results were adjusted to unity. All experimental values were normalized against the latter (Materials and Methods section). Panel B, representative EMSA analysis of interactions between various domains of SMAD3 with NKX2.1 on the SpB promoter. Supershifted nucleoprotein complex with anti-NKX2.1 antibody is indicated with an arrow (Lane 3). Please note that inclusion of GST-LNKR in EMSA does not interfere with formation of nucleoprotein complexes (Lane 8). Asterisks denote P < 0.05.