Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Nov 14;36(1):179–188. doi: 10.1093/nar/gkm871

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Localization of SMAD3 functional domains, which interact with FOXA1. Panel A, mammalian two-hybrid assay was used to test the ability of GAL4-fused MH1, GAL4-fused MH2 or GAL4-fised LNKR domains of SMAD3 to interact with a full-length VP16-fused FOXA1 protein. Results with the empty Gal4 plasmid were adjusted to unity and used to normalize all experimental values (Materials and Methods section). Panel B, representative EMSA analysis of interactions between various domains of SMAD3 with FOXA1 on the SpB promoter. Supershifted nucleoprotein complex with anti-FOXA1 antibody is indicated with an arrow (Lane 3, and a longer exposure in Lane 9). Please note that only GST-LNKR does not interfere with formation of nucleoprotein complexes (Lane 8). Asterisks denote P < 0.05.