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. 2007 Nov 14;36(1):179–188. doi: 10.1093/nar/gkm871

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Chromatin immunoprecipitation (ChIP) analysis of SpB promoter occupancy for NKX2.1 (Panel A) and FOXA1 (Panel B) in response to TGF-beta in MLE15 cells. TGF-beta-treated MLE15 nuclear extracts were immunoprecipitated with either anti-NKX2.1 or anti-FOXA1 antibodies and specific regions of the SpB promoter (Please see Materials and Methods section) were analyzed by PCR. Arrows indicate the PCR products. Lanes 1 are molecular weight markers. Representative results from two independent experiments are shown.