Figure 6.

Effect of nucleolin on mRNA stability. (A) Putative transcription factor binding sites in the SV40 promoter of the pGL3-promoter vector were scanned by the computer program available on the Web site (//transfac.gbf.de/TRANSFAC/index.html). Each putative consensus sequence in the SV40 promoter is enclosed in a box. (B) Putative HuR-binding sites in cPLA₂α 3′UTR are enclosed in boxes. The sequence of transcription stop site is underlined. Schematic diagram of UTR-1-446 vector was presented. (C and D) A549 Cells were transfected with nucleolin or c-Jun siRNA oligonucleotides by lipofection, incubated for 6 h and then transfected with 1 μg of pGL3 (C) and UTR-1-446 (D) vectors. Cells were incubated for 24 h and then the luciferase activities and protein concentrations were determined and normalized. The results shown represent the means ± SEM of three determinations. SC: scramble oligonucleotides. Statistical significance (**P < 0.01 and ***P < 0.001) between c-Jun siRNA and control or nucleolin siRNA and control was analyzed by Student's t-test.