Figure 6.

Characterization of RNA 5′ to the minor strand mRNAs in TbDSS-1 RNAi cells. (A) Schematic of a portion of the T. brucei maxicircle showing major and minor strand genes above and below the line respectively. Black line represents location of probe. (B) Northern hybridization analysis of RNAs containing regions 5′ to the minor strand mRNAs. Thirty micrograms of RNA isolated from uninduced (−tet) and induced (+tet) cells on Day 6 after tetracycline addition was subjected to northern blot analysis with a internally radiolabeled riboprobe. Ethidium bromide staining of ribosomal RNA bands is shown as a loading control.