Table 1.

Summary of unwinding and binding parameters for CRB, DA, and EB

Ligand	φ	φc	Ka, ×106 M−1
CRB	−5.30°	—	0.39
DA	−12.1°	−12°	0.14
EB	−28.7°	−26°	0.02

φ is the unwinding angle calculated by the present method. φ_c is the canonical unwinding angle for a given ligand, obtained as the intercept of a respective isotherm with the ordinate in Fig. 3. K_a is the intrinsic association constant calculated as the negative inverse of a respective isotherm’s slope in Fig. 3.