Table 1.
Ligand | φ | φc | Ka, ×106 M−1 |
---|---|---|---|
CRB | −5.30° | — | 0.39 |
DA | −12.1° | −12° | 0.14 |
EB | −28.7° | −26° | 0.02 |
φ is the unwinding angle calculated by the present method. φc is the canonical unwinding angle for a given ligand, obtained as the intercept of a respective isotherm with the ordinate in Fig. 3. Ka is the intrinsic association constant calculated as the negative inverse of a respective isotherm’s slope in Fig. 3.