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. 2008 Feb 21;118(3):1176–1185. doi: 10.1172/JCI33835

Figure 3. Necrotizing pneumonia in homozygous nmf333 mice following B. cepacia lung infection.

Figure 3

(A) Probability of survival after inoculation with various CFU of B. cepacia (104 CFU, filled squares; 105 CFU, filled triangles; 106 CFU, open circles and filled circles) into the tracheas of WT (open circles; n = 8) and nmf333 (filled circles, filled triangles, filled squares; n = 5 or 6) mice. P = 0.0009 between the 106 CFU infected WT and nmf333 groups; log-rank test. (B) Number of B. cepacia recovered from the lung and spleen homogenates of WT and homozygous nmf333 mice 3.5 days after intratracheal inoculation with 104 B. cepacia, as evaluated by quantitative culture; bacteria were not detected in lung and spleen homogenates from WT mice. Bars represent mean ± SEM (n = 3). **P = 0.029, *P = 0.043; t tests with 2 degrees of freedom. (C) Histopathological appearance of lung and spleen sections obtained from WT and homozygous nmf333 mice 3.5 days after intratracheal inoculation with 104 CFU B. cepacia. Whereas WT tissue samples had minimal or no inflammation, necrotizing pneumonia and splenitis were apparent in the nmf333 strain. Hematoxylin and eosin staining were used. Scale bars: 100 μm.